Before placing the jars in the pressure cooker or pot, you want to make sure the bottom of the jars do not make direct contact with the base of the pressure cooker or pot. Before beginning, its important to assemble all necessarily materials and equipment. My substrate and shrooms are in the fruiting chamber. The vermiculite adds extra space to the grain mixture, allowing the mycelium to extend across the substrate and colonize effectively. Question . The holes have to be bigger than the needle of the syringe. In the mixing bowl, combine 2 cups of vermiculite, 1 tablespoon of gypsum, and 1 cup of water. Is this normal or mold? To achieve this, mist the cakes with a spray bottle 4 times a day until they glisten with moisture. Remove a jar 3. The mycelium grows on an inoculated (spore-infused) substrate until it fully colonizes the medium. If you plan on picking the mushrooms, just grab them from the base with your forefinger and thumb and break it off at the base. Medium: 1.00 2.50 grams Nosh on this delish How To guide. Read here to learn practical tips how to handle a "bad trip". The PF Tek is a great way to grow magic mushrooms, with impressive yields at a very reasonable price. It grew a lot last night so Im hoping to harvest soon. Fill a pan or bowl with a few inches of dry vermiculite. The caps first appear a very dark red color, often covered in very distinct spots (remnants of the universal veil). There should be no space between the lid and the substrate otherwise the syringe needle doesnt reach through the the dry vermiculite to the brown rice flower/vermiculite mix and the spores just sort of pool at the top. Went ahead with inoculating the jars using the oven tek, anyway. Performance & security by Cloudflare. Our guide has everything you need to know about mushroom grow kits from supplies needed to costs. It is one piece, with a removable spray shield. They generally dont fruit as fast as some other strains, but they grow well in suboptimal conditions and produce a large amount of medium-sized fruiting bodies. I've attached pictures of each situation, so hopefully one of you more experienced folks can tell me that everything is fine and just let it go. Once theyre inside, you will have to control temperature and humidity quite closely. It can be a dangerous tool if improperly used. It produces very large, good-quality yields and has a fast colonization rate. Having Sex on Shrooms: Good or Bad Idea?Is having sex on shrooms a good idea or an accident waiting to happen? Avoid totally soaking them and any pools of standing water. Happy growing , Your email address will not be published. Since I've got so many jars growing, I have room for some experimentation. Pressure cooked for 1 hr, cooled over night. Taking a high-level overview, the PF Tek method involves preparing a substrate mixture of brown rice flour, water, and vermiculite. Be sure to wipe up any moisture/substrate on the 1/2" inside and outside the jar. 2 Jars are showing Myc at 4 points (1 showed nothing yesterday, they are small but all 4 inoculation pts. Shake the syringe well, then insert the needle all the way into one of the holes. Try to keep the temperature between 70 and 80F. Oops. Tienen la apariencia clsica de los cubensis, sombreros en tonalidad dorada-amarilla-naranja. Growing Magic Mushrooms, Mushroom spores, Ayahuasca, Magic Mushroom, Cultivation, Magic Mushroom Cultivation, Psilocybe Mushrooms, Cactis and Cannabis, including research, legislation, media coverage, bibliography and lots of links Late to mature, this one doesnt drop spores until the mushrooms are fully grown, making for clean cakes that produce many flushes. Thank you so much for your question. We'll walk you through all you need to know to obtain mushroom spores. Regions with wood chips, rotting logs, or soil rich in plant debris are particularly suitable for growth. The heater just up and quit on me. Good luck! Drill them each roughly two inches apart in a grid pattern. Gold Cap Shrooms Guide: Spores, Effects, IdentificationRead this guide to learn more about the different characteristics of gold cap mushrooms, and how they differ from other psilocybin species. on Step 6. My first flush ever, PF Tek w/ Golden Teacher. They should not be crowded, so you may need multiple fruiting chambers if you have a big batch. Here, the mushrooms will grow over the course of another week or two with consistent misting and fanning. Roll each cake in the dry vermiculite, fully covering each cake. Job Summary. Some say an hour and a half is okay too. Since I'm only inverting this one jar, I will still have the other 9 to use as a control to compare if there is any significant difference. When hyphae from 2 different mycelium bodies meet they share genetic material and create a dikaryotic, secondary mycelium structure from which the fruit bodies (basidiocarps) form.That is why we inject spores in multiple places to create more than 1 mycelium body!The following pictures show progress of the growth. In general, they flourish in the meadows, pastures, and woods of tropical and subtropical regions. Make the hole near the rim, close to the edges. Cultivators love the Golden Teacher spores because they grow in great flushes. Psilocybin Nasal Spray: Relief for Anxiety, PTSD, and DepressionMicrodosing nasal spray with psilocybin, is that possible?! FUCK ME.I dont know what the heck is going on with the pics. Wiped top edge with a clorox wipe, then topped off with a dry layer or vermiculite. I kind of freaked out and picked all the mushrooms and put them in a drying chamber. In the long run, it can become a fun new hobby or a stepping stone to larger-scale grows for a wide variety of mushrooms. Paper towels, however, can be loosely draped over the tops to prevent potential contaminants from falling into the holes. 5 of my 8 original cakes ended up fruiting, and after harvest my total was around 60 wet grams. That was early June. Over time, the water will evaporate and increase the humidity of the chamber. My cakes feel light, and they are definitely not retaining excessive moisture from what I can tell. Lets take a look at some of the pros and cons of this foolproof method. A simple fruiting chamber can be fashioned out of an aquarium or a large tupperware box.The main purpose of the chamber is to create a high humidity (90% to 100%) environment for our mushrooms to flourish.To easily humidify the chamber, fill a strainer with your perlite and soak it in a bowl of water for 5 to 10 minutes, drain it, and line the bottom of the container with it. By way of follow up from this post, after 4 days of the jar being inverted, the mycelium has extended it's coverage by about a quarter of an inch. At least it's good news that all of the jars are growing, in fact one of them has reached the bottom of the jar in a couple of points. Add the wetted perlite to the first layer. @ Craig thank you for taking the time to read! However, other grains require higher temperatures to sterilize properly, so for them you do need a pressure cooker. It strain is very popular due to its easy growth and impressive potency. Top off the jars with dry vermiculite. ago. The water will rehydrate the cakes, increasing the chance of a good flush. Are there any signs if moisture is too high or too low? Place the tight-fitting lid on and let steam for 90 minutes. The sterilization stage is an absolute necessity, but the pressure cooker is not, provided you use brown rice flour as your grain in the substrate. Fanning and misting 3 - 5 times a day has worked well for me. Press question mark to learn the rest of the keyboard shortcuts. It's true that you can overdo the moisture, but I doubt that's going to be an issue for you so I wouldn't worry about that. That is, slide them out of the jars. So, just for the heck of it, I boiled half of my jars for 2 hours. Again, thank you for some great reading. The amount of fresh air is minimal at mycelium growth point, personally I think it has to do more with pressure equalization and the removal of gases produced by the myc growth, rather than osmotic respirations. Jars sterilized and inoculated. My understanding of sterilization is that it opens you up to easier contaminants due to being completely sterile. Then take it out of the water and roll it in dry vermiculite to coat it. You'll find the hobby very addicting, like the rest of us.. Ok, the heat bomb stopped working. It's really good to see your willingness to share your experience here. Read to learn more! Grow kits are ready-to-go packs consisting of the fully-colonized substrate and grow instructions. I went on vacation so the jars had a bit longer to colonize and I wasnt sure if theyd survive. There is also other options such as Dub Tub Tek. Learn how and why the proper storage method is so important. quickly close up & replace lid w/ one equipped w/self-healing injection ports. They originate from fungal spores, the tiny seeds released by the fruiting bodies that are responsible for initiating the mushroom reproduction processes under suitable environmental conditions (more on these conditions later). We would like to refer you to our friends, Grow Sisters, for help with your harvest. Read this article to find out where magic mushrooms grow and more! 1 year ago, 4 years ago Now, flame-sterilize the syringes needle with the lighter. Will order again and again. New Johns Hopkins StudyJohns Hopkins University School of Medicines has just published a new study on psychoactive effects of psilocybin. Canada Approves Psilocybin Treatment for Terminally-Ill Cancer PatientsCanadas Minister of Health, Patty Hajdu approved the use of psilocybin to help ease anxiety and depression of four terminal cancer patients. Taking a high-level overview, the PF Tek method involves preparing a substrate mixture of brown rice flour, water, and vermiculite. Mix thoroughly. This is a wonderful and unique strain that produces very large fruit bodies that are considered to be very potent for a P.cubensis strain. Yeah, that happened! Leave the cakes in the water pot for 24 hours, making sure they stay submerged. Spawn Bags have been inoculated and are looking good. Greetings Fellow Mycologist! Is having sex on shrooms a good idea or an accident waiting to happen? Very thick, meaty stems, dark red caps, and an often-extreme bluing reaction when the fruits are bruised are all notable characteristics of this strain. The strain performs very well both cased and uncased. Plus, the tek requires only commonly available supplies, and no specialized equipment is necessary. Once the mushroom spores land on the optimal medium, it takes four crucial factors to stimulate fruiting body growth: Indoor magic mushroom growing techniques seek to imitate the natural growing conditions that the mushrooms are subjected to in the wild. I think I had at 73f, should have been 75-78. By way of simple experiment, I've decided to try inverting one jar (the one in the foreground of the attached photo) to see if that bottom area may get filled in. Meanwhile, the other 8 jars still have mycelia spreading through them, so I'll just let them keep at it until they start showing pins also. Can't find the strain you looking for, why not add it yourself? - any thoughts? . RH in my apartment is close to 12% so I'm happy I'm able to keep high humidity in the chamber, even though it never rises to much more than the minimum. [i] (2021). Some examples of mushrooms that can be grown with PF tek include: Once a grower has become comfortable using PF tek, graduating to higher-yield methods or methods that work for species that dont like grain will be much easier, since some of the steps are the same. Both the same price for one syringe but apex shipped like 10 days faster , midwest grow kits also shipped quickly, lost 2 blue meanies due to contamination. The Psilocybin Experience: What's the Deal With Magic Mushrooms? The holes provide constant fresh air exchange. If youre already experienced with PF Tek you might want to look into WBS Tek which is a bit more complicated but tends to yield more. My question is: can I reuse the un-inoculated mix to re-pack and sterilize the jars. Did you make this project? For the hunting, growing, and consuming of mushrooms. It is a slow maturer and grows best with the BRF or PF Tek method. Then, sterilize this space by wiping with 70% iso alcohol. Sorry for the upside down photo, I don't know why that happened in the post, the original is not. 5 years ago Space is limited in my apartment and I don't have tons of space for another large tote. Oregon's Initiative to Legalize Mushrooms | Initiative Petition 34Oregon continues to push ahead with their initiative to legalize Psilocybin in 2020. Five days in right now. How to Grow Magic Mushrooms: Step-by-StepThis step-by-step guide will show you how to grow magic mushrooms at home. I only have a small pressure cooker. With the abundance of information and materials available online, growing psilocybin mushrooms has never been more accessible and easy. Happy growing! Bad Trips: Practical Tips for Challenging JourneysPsychedelic experiences can be exciting but some can be challenging. It is an all round excellent magic mushroom that wont leave anything to be desired. When drilling, dont press too hard or the container may crack. Yeah that happens, the myc will try to colonize the verm layer, no worries there. It is when your cakes are done growing the mycelium, then you give them some diffused light daily, some folks like ot shock the cakes by soaking in cold water. From here, the rehydrated cake can be placed back in the SGFC for another flush. One of the major advantages of the PF Tek method is that the materials needed are cheap and widely available online or in grocery and gardening stores. Haha, like I said, tried I tell ya I dont think a week has ever felt longer than when I was waiting for my jars to colonize haha. Come find out the supplement industry's dirty secret. Ok I finally got a chance to sit back down here after checking all the Jars throughly. Psilocybin mushrooms (of which there are over 200 species) can be found on every major continent. Disclaimer: Psilocybin is potentially categorized as an illegal drug. Incubating the jars means letting them sit in a warm, dark place as the mycelium colonizes the substrate. I've been lurking here for a while and I doubt I would have ever made it this far without all of you :), "I put them in a closet and did my best to forget about them" Update comes next day lol. Golden Teacher mushrooms have a mild to moderate potency, and the recommended dosage is between 1 gram and 2.5 grams, whereas most people take 2 grams to feel some effects but nothing too intense. See the second picture on this step for an example of contamination, green penicillium mold is a common contaminant. Santa was a Mushroom-Eating ShamanSanta Claus exists in many cultures, and his roots are not only pagan. Thank Hippie, RH is stays at about 68% inside the grow chamber. Once the fungus has fully colonized the mixture, the contents of the jar can be slid out easily, held together in a block or cake by the fungal mycelium. You can email the site owner to let them know you were blocked. Teacher's insanely racist YouTube rant is a product of woke culture. Sterilize until it glows red hot, but be sure to avoid the part of the needle nearest to the plastic base of the syringe. I just popped another cake out that had been showing a couple pins and the top had several, up to an inch long! It was developed by Robert 'Billy' McPherson, an American fungus fan who went by the name Psylocybe Fanaticas, and shared his eponymous growing instructions with the world. To prevent breakage, be careful not to heat them up too quickly and to let them cool slowly by leaving them in the steamer until it cools fully. Paul Stamets Profile: Mushroom Guru, Filmmaker, Nutritionist, Scientist, Microdosing Psilocybin & Common Dosage Explained, Psilocybin Nasal Spray: Relief for Anxiety, PTSD, and Depression, Mazatec Mushroom Usage: Notes on Approach, Setting and Species for Curious Psilonauts, Mara Sabina: The Mazatec Magic Mushroom Woman, Kilindi Iyi: Mycologist, Traveler, Teacher, Shroom Chocolate Recipes: How to Make Magic Mushroom Chocolates, Why Do People Use Psilocybin? To elevate the jars, place a small, thin towel on the base, and arrange the jars on top of that. Dont open up the pressure cooker or pot until this is done. The vermiculite adds extra space to the grain mixture, allowing the mycelium to extend across the substrate and colonize effectively. 22 days ago. Fill the bottom with 1-2 inches of dry perlite, making sure the entire base is evenly coated. on Step 8, 80F-86F is a good way to promote bacterial growth. How to Buy Psilocybin SporesInterested in psilocybin mushrooms? They sure did! That effort being the importance of keeping the developing cakes completely dark. Second and third flush fruits can become enormous, often peaking at heights of nine inches. Magic mushroom spores, the most important piece of the grow, are needed to inoculate the substrate. I ordered 1 syringe of golden teacher spores from spore works, and they sent me an extra syringe of the chitwan strain. Inves in a small food grinder, to grind the seed, it doesn't take all that long. Latest Updates. Photos: The San Gabriel Valley Bowl All Star Game. With a tissue or your fingertip, wipe the insides of the jar down to the substrate. I also still have my 3 other jars at about 95% colonization, and I will allow them to sit for the full week to consolidate before adding to my chamber. Golden Teacher run on PF Tek Started By joeya , Apr 17 2019 12:20 PM Page 1 of 2 1 2 Next Please log in to reply 33 replies to this topic #1 joeya Mycotopiate Black VIP 534 posts Posted 17 April 2019 - 12:20 PM Today marks the 2 week point on my PF TEK run of Golden Teacher. Psilocybin Guide: Effects, Common Uses, SafetyOur ultimate guide to Psilocybin has everything you want to know about this psychedelic fungi from its uses to its legal status. Drinking glasses of appropriate size work well, too, though in that case youll have to improvise a lid. Leave a 1/2 to 3/4 inch space at the top. Very useful information, across the board! Place a plate or pan on top of the cakes to totally submerge them (they will float and not fully submerge otherwise). Did the same, works just fine for me, too. Once all of the jars are inoculated, place them in a dark tote or on a shelf in a closet without direct sunlight (ambient light is fine). During steaming, add extra water as needed so the pot doesnt run dry. If holds true for me, this is good info, as it can be a pain setting things up to reliably maintain that 80-86. Thank you so much for the input! One has a small cap coming in and the rest are currently inactive. Fruits do not form as abundantly as some other strains, however the size compensates in dried weight for the lack in numbers. Although its not the highest-yielding method available, it is among the most reliableand its simple enough that beginners can almost always realize a harvest. Leave the jars in the pressure cooker until you are ready to Inoculate. Do not tap down or tamp down the substrate. PF tek is very popular among Psilocybe growers (thats the vaunted magic mushroom), but will also work with any legal, culinary mushroom able to grow on grain. The "golden teacher" is one of our favorites. The recommended dose for dried Golden Teacher mushrooms is between 1gram and 2.5grams. Dunno if the last pic posted correctly or not. Beginners looking to grow magic mushrooms may find magic mushroom grow kits are an attractive option for their ease and simplicity. For most experienced shroomers, this dose may be quite small but this allows a margin of error for novice shroomers who never ingested Golden Teachers before. The action you just performed triggered the security solution. Just found one problem when I got to inoculating my jars. First flush. Alternatively, if I did something wrong to cause this, I would like to know as well. I am a beginner, I followed instructions, I received spore in syringes, it was pinkish in color. The half inch of dry vermiculite needs to end at the very brim of the jar. I've read to keep different strains in different fruiting chambers, other than mixing of spores, is this something I should try to stay away from? If a mod can advise me on how to put pics in the actual posting, rather than the thumbnail attachment, it would be greatly appreciated!! Really informative article thanks Austin. Average mature fruit bodies reach heights of over five inches with caps often spanning two inches in diameter. I can post pictures in the comments of any part of my grow if it will help. Note: At this point, the colonized substrate (cakes) are mostly resistant to contamination, so strict sterile technique isnt needed. The foil is to keep any water from getting in through the holes during sterilization. Close up your cooker and put it on a stove. Read to learn about how the U.S. is moving to decriminalize psilocybin. Shroom Chocolate Recipes: How to Make Magic Mushroom ChocolatesThis recipe provides step by step directions on how you can make mushroom chocolates with the necessary ingredients. It doesnt do to get halfway through and realize some important component is missing! Add cold water to the pot. Read here to learn more. Products were as specified and lead times were as expected! The Psilocybin Experience: What's the Deal With Magic Mushrooms?From microdoses to macrodoses, the psilocybin experience has been sought after both medicinally and recreationally for millennia. We must, therefore, sterilize the substrate before inoculating the substrate with the mushroom spores. Could mushrooms be trying to teach humans something as well? Repeat this process for each jar. The PF classic strain is named after Robert McPherson, aka Psylocybe Fanaticus who pioneered the PF Tek growing method. One of those pins has doubled in size, so I have a slight possibly of developing an early sample shroom from this crop! The sharp edge around the hole can tear the foil in the next few steps. This website is using a security service to protect itself from online attacks. Two of my cakes developed a few pins, so I have removed them from their jars, cleaned and soaked, and now in a fruiting chamber! I also meant to order regular GTs but bought the leucistic by accident. The added perlite soaks up the water in the air and provides a steady stream of high relative humidity. I've noticed in pictures on here, as well as my own grow, that the bottom of the jar does not seem to grow mycelia, so I got to thinking about why that may be. Once each cake it out, rinse it off and then soak it in cold water for about 20 hours. This guide is intended to give educational content and should in no way be viewed as medical recommendations. This is a fun hobby and Im so excited to share with you all! Do not light from inside the chamber, or the cakes may get scorched. How to Make Shroom Tea: Best Recipe and DosageA step by step guide on how to brew shroom tea, and why entheogenic psilocybin tea is a preferred method for psychedelic connoisseurs. Those 1/2 pint tall(reg mouth) jars sometimes wont colonize all the way to the bottom, if they are stalled and you can't get them to restart, you can birth them if the part that is colonized has been long enough to be consolidated, just cut off the uncolonized portion with a clean blade. Does anyone have any input on how to handle the misting and fanning in such dry conditions? The first flush will be ready to harvest right before the partial veil (the thin membrane below the cap) breaks. Set the SGFC over four stable objects to elevate it at least two inches (this allows gas exchange from the bottom). I spend a lot of time researching different strains and hope to have a nice spore print selection going forward. Additionally healing-mushrooms.net is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to amazon.com. Golden Teacher - Psilocybe cubensis. Golden Teachers are fairly new and they first appeared during the 1980s. One has a small cap coming in and the rest are currently inactive. Thank you JK, glad to hear it. Well, my first two cakes have a nice flush of pins developing on them now. 7. Like in a cupboard above the refridgerator, or in a cardboard box by your computer tower.This allows the spores to incubate into mycelium the body that absorbs nutrients and water.Keep them around 80-86 degrees F. It will just take longer for the mycelium to grow if it isn't near the optimum temperature.It takes around 3 to 4 days to see the first hairs of mycelium forming as white spots and 3 to 5 weeks to let the fungus get to a 100% foothold in all the substrate, depending on temperature conditions. Repeat for the other three holes, totaling about 1cc of spore solution per jar. Mushly. If you're comfortable it's fine for the jars. Here is the basic order of things during the Inoculation procedure: 1. I suspect that it will only be a few days until the most advanced couple of jars are ready to birth, although I'm hoping to hold off until after the weekend, as I'll be out of town for a few days. Ever wondered why some Mushroom Supplements are so cheap? r/MushroomGrowers is a supportive community of amateurs and professionals from around the world collaborating on mushroom cultivation. They will first appear as tiny bumps that will grow into pins. The information found on healing-mushrooms.net is strictly the author expressing an opinion. 3 years ago Inject approximately cc ( mL) of spore solution. *DELETED*. Mush Love . This dose may be too small for any significant psychedelic effect for the more experienced shroomers, but it is enough for the inexperienced or . I've never heard or read anything about keeping strains separate and I've never bothered to do it myself. This instructable will go over one of the most basic grow techniques (PF Tek) for growing a wide variety of mushrooms including Shaggy Mane, Lion's Mane, and P. Cubensis. Each day I wake up eager to see their growth. Are there different types of psilocybin? I'm currently in the process of trying my hand at growing mushrooms for the first time, and I have a couple questions. You can cut or gently twist the mushrooms close to the cake to remove them. Now that the hard part is over, you just need to let the jars rest somewhere warm and dark. :gah. When I touch the rolled layer its bone dry and there is some falling off onto the foil down below as well. It is known to be a steady, slow grower, but with the payoff of a powerful trip in the end. 1/21- BRF substrate created and filled into jars. Waterproof medical tape over the pre punched holes, double layer of aluminum foil. Worked fine. Keep doing what you're doing. Psilocybin and Magic Mushroom ResourcesCurious to learn more about psilocybin? Just steam the jars instead. Very grateful for their forgiveness. It can be longer or shorter, though. This will take approximately 2-3 weeks. Sterilize the jars and their contents by giving them a steam-bath for an hour and a half. 1. And psychonauts love them because the psychedelic journey of these mushroom are known to have a profound impact. Yes, pink is contamanation. Product is as described. Mostly you just want to make sure you keep the perlite moist so that the humidity stays up and the cakes don't dry out, which you seem to have under control already. FLushing and fruiting are basically the same thing. so far. Essential Duties and Responsibilities. Even if that doesn't work out, at least I will have a small dubtub set up ready for my next grow. Read our guide to learn about the different magic mushroom strains and their individual effects. Oregan a start-up Silo Wellness believes so and has created this new option for PTSD treatment. Be sure not to compress the mix into the jars. It can help to mark out the holes with a marker prior to drilling. Having the correct amount of moisture in the mix is called field capacity, and getting it right is important for a good grow. Looks like I'm off and running now. Once the cakes are on top of the foil, mist the chamber with a spray bottle and fan the chamber with the lid before closing. You may just need to give it some more time. Add the mixture into the 5 canning jars equally, filling to inch below the rims. We all may have Mara Sabina to thank for exposing the Western world to the healing properties of the psilocybin mushroom. Once the 60 or 90 minutes is up (for the pressure cooker or pot, respectively), turn off the heat and let them cool for 8-10 hours. sad :/. But to begin fruiting after the mycelium has fully populated the cakes, subdued light for part of every day for a week or so until fruiting begins. How to Make Shroom Tea: Best Recipe and Dosage, R. Gordon Wasson: Author and Mushroom Expert, Shrooms vs Acid: Differences and Similarities Explained, Quantum Mechanics, Reality, and Magic Mushrooms, Psilocybin Guide: Effects, Common Uses, Safety. 75f for fruiting (there not picky, wil fruit 69F-80 possibly higher) plenty of fresh air. Why Do People Use Psilocybin? Use something in the bottom of the pot to keep the jars out of the water. You know you can tip the jar a bit to get the syringe to fit in the clean box. yet people out here taking 10+ grams and going on a rampage. Mix the vermiculite and water so that the vermiculite is fully saturated but does not drip much. Watch the temperature, just remember that the jars will be a few degrees warmer than room temperature, you might see faster colonization but conversely you might see mold too :special: tyvek will exchange enough air as not much is needed. Inoculation is the process of introducing spores of your chosing into the sterilized substrate to take hold and grow. Mush love . Rinse the cakes under cold running water to remove the dry vermiculite layer.